Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris
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چکیده
منابع مشابه
Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris
The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0058393